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KMID : 0603820140200030129
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Journal of Experimental & Biomedical Science
2014 Volume.20 No. 3 p.129 ~ p.138
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Inhibitory Effects of Water Extract from Rice Bran Due to cAMP-dependent Phosphorylation of VASP (Ser157) on ADP-induced Platelet Aggregation
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Kim Hyun-Hong
Hong Jeong-Hwa
Pajaree Ingkasupart
Lee Dong-Ha
Park Hwa-Jin
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Abstract
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In this study, we investigated the effect of water extract from rice bran (RB) on ADP (20 μM)-stimulated platelet aggregation. RB dose-dependently inhibited ADP-induced platelet aggregation, and its IC50 value was 224.0 μg/mL, which was increased by adenylate cyclase inhibitor SQ22536 and cAMP-dependent protein kinase (A-kinase) inhibitor Rp-8-Br-cAMPS. RB elevated the phosphorylation of VASP (Ser157) which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that RB-elevated cAMP contributed to the phosphorylation of VASP (Ser157) to inhibit ADP-induced platelet aggregation. Therefore, we demonstrate that RB has an antiplatelet effect via cAMP-dependent phosphorylation of VASP (Ser157), and RB may have preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.
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KEYWORD
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Rice bran, Platelet aggregation, cAMP-dependent protein kinase, cAMP, Vasodilator-stimulated phosphoprotein-Ser157 phosphorylation
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